Difference between revisions of "Part:BBa K3128009:Design"

 
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===Design Notes===
 
===Design Notes===
−
To exchange the RFP gene by another gene  
+
To exchange the RFP gene by another gene two restrictions sites were added on both sides of the RFP gene : 5'_BglII_RFP_BamHI_3'
−
two restrictions sites were added on both sides of the RFP gene : 5'_BglII_RFP_BamHI_3'
+
  
  

Revision as of 07:49, 16 September 2019


RFP protein under PLac promoter (with two restriction sites around the reporter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 229
    Illegal BamHI site found at 913
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 789
    Illegal AgeI site found at 901
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To exchange the RFP gene by another gene two restrictions sites were added on both sides of the RFP gene : 5'_BglII_RFP_BamHI_3'


Source

iGEM plate

References