Difference between revisions of "Part:BBa K3128008:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K3128008 short</partinfo>
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<partinfo>BBa_E1010 short</partinfo>
  
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<partinfo>BBa_K3128008 SequenceAndFeatures</partinfo>
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<partinfo>BBa_E1010 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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This is a twin of BBa_E1010 with restriction sites added on both sides.
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TAATAA double stop codon added (DE).
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5'BglII_RFP_BamHI3'
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Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
  
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From <cite>Campbell</cite>:
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The final clone, designated mRFP1, contains a total of 33 mutations relative to DsRed of which 13 are internal to the &beta;-barrel (N42Q, V44A, V71A, K83L, F124L, L150M, K163M, V175A, F177V, S179T, V195T, S197I, and T217A). Of the 20 remaining external mutations, three are the aggregation-reducing mutations from T1 (R2A, K5E, and N6D), three are AB interface mutations (I125R, V127T, and I180T), ten are AC interface mutations (R153E, H162K, A164R, L174D, Y192A, Y194K, H222S, L223T, F224G, and L225A), and four are additional beneficial mutations (T21S, H41T, C117E, and V156A).
  
 
===Source===
 
===Source===
 +
[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837 Genbank accession AF506027]
  
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iGEM plates
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<biblio>
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#Campbell pmid=12060735
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</biblio>
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[http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735 URL]
  
 
===References===
 
===References===
 +
<biblio>
 +
#Zhang pmid=12461557
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</biblio>

Revision as of 07:04, 16 September 2019

**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TAATAA double stop codon added (DE).

Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.

From Campbell: The final clone, designated mRFP1, contains a total of 33 mutations relative to DsRed of which 13 are internal to the β-barrel (N42Q, V44A, V71A, K83L, F124L, L150M, K163M, V175A, F177V, S179T, V195T, S197I, and T217A). Of the 20 remaining external mutations, three are the aggregation-reducing mutations from T1 (R2A, K5E, and N6D), three are AB interface mutations (I125R, V127T, and I180T), ten are AC interface mutations (R153E, H162K, A164R, L174D, Y192A, Y194K, H222S, L223T, F224G, and L225A), and four are additional beneficial mutations (T21S, H41T, C117E, and V156A).

Source

[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=nucleotide&dopt=GenBank&list_uids=21464837 Genbank accession AF506027]

<biblio>

  1. Campbell pmid=12060735

</biblio> [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735 URL]

References

<biblio>

  1. Zhang pmid=12461557

</biblio>