Difference between revisions of "Part:BBa K2959002:Design"

 
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===Design Notes===
 
===Design Notes===
−
The sequence consists in a truncated version of Erv1p that lacks the first 72 amino acids of the full sequence. It has been proved that this version shows similar or even improved sulfhydryl oxidase activity.
+
The sequence consists in a truncated version of Erv1p that lacks the first 72 amino acids of the full sequence. It has been proved that this version shows similar or even improved sulfhydryl oxidase activity<sup>1</sup>.
  
  
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===Source===
 
===Source===
  
−
The full amino acid sequence was reported by Lisowsky (1996). The truncated Erv1p amino acid sequence was retrieved from Lee et al. (2000).
+
The complete amino acid sequence was reported by Lisowsky (1996). The truncated Erv1p amino acid sequence was retrieved from Lee et al. (2000).
  
 
===References===
 
===References===
 +
1. Lee, J. E., Hofhaus, G., & Lisowsky, T. (2000). Erv1p from Saccharomyces cerevisiae is a FAD‐linked sulfhydryl oxidase. FEBS letters, 477(1-2), 62-66.
 +
<br>
 +
2. Lisowsky, T. (1996). Removal of an intron with unique 3′ branch site creates an amino‐terminal protein sequence directing the scERV1 gene product to mitochondria. Yeast, 12(15), 1501-1510.

Revision as of 22:38, 15 September 2019


Truncated Saccharomyces cerevisiae FAD-linked sulfhydryl oxidase Erv1p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 58
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence consists in a truncated version of Erv1p that lacks the first 72 amino acids of the full sequence. It has been proved that this version shows similar or even improved sulfhydryl oxidase activity1.


Source

The complete amino acid sequence was reported by Lisowsky (1996). The truncated Erv1p amino acid sequence was retrieved from Lee et al. (2000).

References

1. Lee, J. E., Hofhaus, G., & Lisowsky, T. (2000). Erv1p from Saccharomyces cerevisiae is a FAD‐linked sulfhydryl oxidase. FEBS letters, 477(1-2), 62-66.
2. Lisowsky, T. (1996). Removal of an intron with unique 3′ branch site creates an amino‐terminal protein sequence directing the scERV1 gene product to mitochondria. Yeast, 12(15), 1501-1510.