Difference between revisions of "Part:BBa K3128001:Design"
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===Design Notes=== | ===Design Notes=== | ||
To do a BACTH assay the reporter gene need to : | To do a BACTH assay the reporter gene need to : | ||
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Have a CAP inducible promoter : PLac | Have a CAP inducible promoter : PLac | ||
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A strong RBS to optimize protein expression | A strong RBS to optimize protein expression | ||
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A easly measurable protein : NanoLuciferase | A easly measurable protein : NanoLuciferase | ||
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https://2019.igem.org/wiki/images/thumb/7/70/T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png/595px-T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png | https://2019.igem.org/wiki/images/thumb/7/70/T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png/595px-T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png | ||
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===Source=== | ===Source=== | ||
Revision as of 19:30, 15 September 2019
NanoLuciferase reporter for BACTH assay
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To do a BACTH assay the reporter gene need to :
Have a CAP inducible promoter : PLac
A strong RBS to optimize protein expression
A easly measurable protein : NanoLuciferase
Source
iGEM plate : Bba_J04450 and Bba_K1680009