Difference between revisions of "Part:BBa K3128001:Design"

 
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A strong RBS to optimize protein expression
 
A strong RBS to optimize protein expression
 
A easly measurable protein : NanoLuciferase
 
A easly measurable protein : NanoLuciferase
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https://2019.igem.org/wiki/images/thumb/7/70/T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png/595px-T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png
  
  

Revision as of 19:29, 15 September 2019


NanoLuciferase reporter for BACTH assay


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To do a BACTH assay the reporter gene need to : Have a CAP inducible promoter : PLac A strong RBS to optimize protein expression A easly measurable protein : NanoLuciferase


595px-T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png


Source

iGEM plate : Bba_J04450 and Bba_K1680009

References