Difference between revisions of "Part:BBa K3128004:Design"

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<partinfo>BBa_K3128001 short</partinfo>
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<partinfo>BBa_K3128004 short</partinfo>
  
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<partinfo>BBa_K3128001 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3128004 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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https://2019.igem.org/wiki/images/thumb/7/70/T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png/595px-T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png
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BBA J04450 was used for its CAP dependant Lactose Promoter (PLac) and it's strong RBS making it able to produce a large amount of protein when activated by IPTG and cAMP-CAP complexes.
 
BBA J04450 was used for its CAP dependant Lactose Promoter (PLac) and it's strong RBS making it able to produce a large amount of protein when activated by IPTG and cAMP-CAP complexes.
 
Two restrictions sites were added on the biobrick to to remove the RFP gene and replace it by the NanoLuciferase gene, BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.
 
Two restrictions sites were added on the biobrick to to remove the RFP gene and replace it by the NanoLuciferase gene, BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.

Revision as of 17:59, 15 September 2019


NanoLuciferase reporter for BACTH assay (with two restriction enzymes around the reporter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 229
    Illegal BamHI site found at 769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

595px-T--Grenoble-Alpes--Plasmid_PLacNanoLuc.png

BBA J04450 was used for its CAP dependant Lactose Promoter (PLac) and it's strong RBS making it able to produce a large amount of protein when activated by IPTG and cAMP-CAP complexes. Two restrictions sites were added on the biobrick to to remove the RFP gene and replace it by the NanoLuciferase gene, BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.

Source

PLac-RBS and the termintors are from iGEM plates from Bba_J04450. NanoLuc gene is from Promega vector's pNL1.1[Nluc]

References