Difference between revisions of "Part:BBa I746916:Experience"

Revision as of 15:33, 13 September 2019

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Applications of BBa_I746916

2019 iGEM team Linkoping Sweden

Figure 1. 1 liter growth medium containing induced CBD-sfGFP BL21 (DE3) on a UV-table.

The picture to the left is a 1 liter culture of induced CBD-sfGFP e.coli BL21(DE3).

Pictures above shows BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP at different IPTG concentrations between 0.05 - 1.0 mM. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.coli suggesting that the induction windows for expressing CBD-sfGFP are different between E.coli BL21 (DE3) and Vibrio natriegens Vmax.

User Reviews

UNIQ16716f1fd5684402-partinfo-00000000-QINU UNIQ16716f1fd5684402-partinfo-00000001-QINU

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 The picture to the left is a 1 liter culture of induced CBD-sfGFP e.coli BL21(DE3).
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 <br> Pictures above shows BL21 DE3 and Vibrio natriegens Vmax expressing CBD-sfGFP at different IPTG concentrations between 0.05 - 1.0 mM. The induction gradient for Vibrio is more correlated with the IPTG concentration then E.coli suggesting that the induction windows for expressing CBD-sfGFP are different between E.coli BL21 (DE3) and Vibrio natriegens Vmax.
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