Difference between revisions of "Part:BBa K2992015"
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<partinfo>BBa_K2992015short</partinfo> | <partinfo>BBa_K2992015short</partinfo> | ||
| − | 5’-UTR containing RBS for <i>ntnH</i> gene from <i>C. botulinum</i> | + | 5’-UTR containing RBS for <i>ntnH</i> gene from <i>C. botulinum</i> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 17:18, 12 September 2019
No part name specified with partinfo tag.
5’-UTR containing RBS for ntnH gene from C. botulinum
Usage and Biology
The native 5’-UTR containing the RBS is found naturally upstream of ntnH in C. botulinum. ntnH encodes the non-toxic non-haemagglutinin component of the botulinum neurotoxin complexes. Its expression is directly regulated by BotR. In our project we use the regulatory region of ntnH to drive expression of the toxin regulator botR and our chosen reporter genes of interest which have been placed under the control of the neurotoxin and neurotoxin-associated promoters. Doing so allows us to link volatile reporter production with neurotoxin production following food manufacturing processes.
Characterisation
Data incoming.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Raffestin et al., 2005 (update)