Difference between revisions of "Part:BBa K2951007:Design"

 
(→‎Design Notes)
 
Line 9: Line 9:
 
1.Codon optimization:
 
1.Codon optimization:
 
We optimized the sequence's codon usage for E.coli.
 
We optimized the sequence's codon usage for E.coli.
 +
 
2.RFC 10 compatible:
 
2.RFC 10 compatible:
 
We checked for restriction sites for EcoRI, XbaI, SpeI, PstI and NotI in order to make it RFC compatible.
 
We checked for restriction sites for EcoRI, XbaI, SpeI, PstI and NotI in order to make it RFC compatible.
 +
 
3.Deletion of transmembrane domain:
 
3.Deletion of transmembrane domain:
 
To improve the solubility of hemagglutinin, we predicted the transmembrane domain with TMHMM Server v. 2.0.
 
To improve the solubility of hemagglutinin, we predicted the transmembrane domain with TMHMM Server v. 2.0.
−
 
−
 
  
 
===Source===
 
===Source===

Latest revision as of 17:45, 11 September 2019


Influenza A hemagglutinin with poly linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 291
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1.Codon optimization: We optimized the sequence's codon usage for E.coli.

2.RFC 10 compatible: We checked for restriction sites for EcoRI, XbaI, SpeI, PstI and NotI in order to make it RFC compatible.

3.Deletion of transmembrane domain: To improve the solubility of hemagglutinin, we predicted the transmembrane domain with TMHMM Server v. 2.0.

Source

The source of this part is from Influenza A virus (A/WSN/1933 TS61(H1N1)) segment 4, complete sequence, gene:20-1717 The sequence was obtained from NCBI(https://www.ncbi.nlm.nih.gov/nucleotide/CY010788.1?report=genbank&log$=nuclalign&blast_rank=2&RID=F439K4B5015)

References