Difference between revisions of "Part:BBa K2992011"

 
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RBS region for the <i>botR</i>  gene in <i>C. botulinum</i>   
 
RBS region for the <i>botR</i>  gene in <i>C. botulinum</i>   
  
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===Usage and Biology===
 
===Usage and Biology===
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This part entry represents the RBS from the alternative sigma factor-encoding gene <i>botR</i>. BotR is a positive regulator of neurotoxin production. In our project we use the <i>botR</i> RBS as part of the whole regulatory region for the expression of <i>botR</i> comprising P<i>botR</i> including the 5’-UTR (add number) this entry, the RBS, and the <i>botR</i> gene (BBa_K2992002 <haref=” https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2018&group=Nottingham>. The RBS was also used in conjunction with <i>botR without its native promoter to polar expression from the <i>pyrD</i> and <i>pyrE</i> genes on the <i>C. sporogenes</i> genome. <br><br>
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===Characterisation===
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Data incoming
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2992011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992011 SequenceAndFeatures</partinfo>
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===References===
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Incoming
  
  

Revision as of 09:04, 10 September 2019


RBS region for botR gene from C. botulinum

RBS region for the botR gene in C. botulinum


Usage and Biology

This part entry represents the RBS from the alternative sigma factor-encoding gene botR. BotR is a positive regulator of neurotoxin production. In our project we use the botR RBS as part of the whole regulatory region for the expression of botR comprising PbotR including the 5’-UTR (add number) this entry, the RBS, and the botR gene (BBa_K2992002 <haref=” https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2018&group=Nottingham>. The RBS was also used in conjunction with botR without its native promoter to polar expression from the <i>pyrD and pyrE genes on the C. sporogenes genome.

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Incoming