Difference between revisions of "Part:BBa K3020000:Experience"
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Typical DNA damage agents include nalidixic acid (NA) (Sigma), formaldehyde (formaldehyde, CH2O) (Sigma) and hydrogen peroxide(H2O2).The following non-DNA damage reagent was used as a negative control:kanamycin sulfate，KAN）（Amresco）,acetone,phenol. | Typical DNA damage agents include nalidixic acid (NA) (Sigma), formaldehyde (formaldehyde, CH2O) (Sigma) and hydrogen peroxide(H2O2).The following non-DNA damage reagent was used as a negative control:kanamycin sulfate，KAN）（Amresco）,acetone,phenol. | ||
The reagent concentration and gradient were calculated and added to the 40 ml bacterial solution (LB) system according to the following induction volume. | The reagent concentration and gradient were calculated and added to the 40 ml bacterial solution (LB) system according to the following induction volume. | ||
| − | [[File:BIT201906-youdaoji1.jpeg|thumb| | + | [[File:BIT201906-youdaoji1.jpeg|thumb|800px|center|'''DNA damage agent concentration gradient and induced addition''']] |
| − | [[File:BIT201907-youdao2.jpeg|thumb| | + | [[File:BIT201907-youdao2.jpeg|thumb|800px|center|'''non-DNA damage agent concentration gradient and induced addition''']] |
Revision as of 08:10, 10 September 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3020000
User Reviews
Typical DNA damage agents include nalidixic acid (NA) (Sigma), formaldehyde (formaldehyde, CH2O) (Sigma) and hydrogen peroxide(H2O2).The following non-DNA damage reagent was used as a negative control:kanamycin sulfate，KAN）（Amresco）,acetone,phenol. The reagent concentration and gradient were calculated and added to the 40 ml bacterial solution (LB) system according to the following induction volume.
