Difference between revisions of "Part:BBa K2992009"

Revision as of 15:33, 9 September 2019

Synthetic guide RNA sequence 1 for botR integration

Synthetic guide RNA sequence 1 for the integration of several botR constructs at the pyrE locus of the C. sporogenes genome

Usage and Biology

Synthetic guide RNA sequence 1 for the use of CRISPR-Cas9 for genomic integration of various botR constructs in the generation of our volatile reporter strains for predicting neurotoxin production following food manufacture. Genomic integration of these constructs was at the pyrE locus of C. sporogenes.

Characterisation

Data incoming

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Incoming.

@@ Line 5: / Line 5: @@
 Synthetic guide RNA sequence 1 for the integration of several <i>botR</i> constructs at the <i>pyrE</i> locus of the <i>C. sporogenes</i> genome
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Synthetic guide RNA sequence 1 for the use of CRISPR-Cas9 for genomic integration of various botR constructs in the generation of our volatile reporter strains for predicting neurotoxin production following food manufacture. Genomic integration of these constructs was at the <i>pyrE</i> locus of <i>C. sporogenes</i>. <br><br>
+===Characterisation===
+Data incoming <br><br>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
@@ Line 13: / Line 16: @@
+===References===
+Incoming.
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K2992009 parameters</partinfo>
 <!-- -->