Difference between revisions of "Part:BBa K2992007"

 
Line 5: Line 5:
 
The A and B sub-units of the CtfAB complex are encoded in an operon on the genome of <i>C. botulinum</i>. This part entry represents the intergenic region immediately downstream of the stop codon for <i>ctfA</i> and upstream of the start codon for <i>ctfB</i>. This region also contains part of the RBS for <i>ctfB</i>.  
 
The A and B sub-units of the CtfAB complex are encoded in an operon on the genome of <i>C. botulinum</i>. This part entry represents the intergenic region immediately downstream of the stop codon for <i>ctfA</i> and upstream of the start codon for <i>ctfB</i>. This region also contains part of the RBS for <i>ctfB</i>.  
  
−
<!-- Add more about the biology of this part here
+
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
The CtfAB complex is central to the production of biotechnologically relevant solvents in <i>C. acetobutylicum</i> such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. the CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both <i>C. acetobutylicum</i> and <i>C. botulinum</i>-derived <i>ctfAB</i>. <br><br>
 +
 +
===Characterisation===
 +
Data incoming <br><br>
  
 
<!-- -->
 
<!-- -->
Line 12: Line 16:
 
<partinfo>BBa_K2992007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2992007 SequenceAndFeatures</partinfo>
  
 +
===References===
 +
Incoming.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 13:44, 9 September 2019


intergenic region between ctfA and ctfB from C. botulinum

The A and B sub-units of the CtfAB complex are encoded in an operon on the genome of C. botulinum. This part entry represents the intergenic region immediately downstream of the stop codon for ctfA and upstream of the start codon for ctfB. This region also contains part of the RBS for ctfB.


Usage and Biology

The CtfAB complex is central to the production of biotechnologically relevant solvents in C. acetobutylicum such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. the CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both C. acetobutylicum and C. botulinum-derived ctfAB.

Characterisation

Data incoming

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Incoming.