Difference between revisions of "Part:BBa K3257045"
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LacI is one of genes in Lac operon encoding the inhibitor protein binding to LacO (cis-acting element). In response of IPTG, the inhibitor protein detaches from LacO and enables the transcription of downstream genes. We mutated some specific sites in LacI gene to improve its sensibility to IPTG.[2] Using EGFP as a reporter, its fluorescence intensity appears a greater leap before and after the induction of IPTG. Also, we induce the improved Lac operon by arabinose to verify its orthogonal response to IPTG. | LacI is one of genes in Lac operon encoding the inhibitor protein binding to LacO (cis-acting element). In response of IPTG, the inhibitor protein detaches from LacO and enables the transcription of downstream genes. We mutated some specific sites in LacI gene to improve its sensibility to IPTG.[2] Using EGFP as a reporter, its fluorescence intensity appears a greater leap before and after the induction of IPTG. Also, we induce the improved Lac operon by arabinose to verify its orthogonal response to IPTG. | ||
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<partinfo>BBa_K3257045 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3257045 SequenceAndFeatures</partinfo> | ||
Revision as of 11:25, 9 September 2019
Improved LacI
LacI is one of genes in Lac operon encoding the inhibitor protein binding to LacO (cis-acting element). In response of IPTG, the inhibitor protein detaches from LacO and enables the transcription of downstream genes. We mutated some specific sites in LacI gene to improve its sensibility to IPTG.[2] Using EGFP as a reporter, its fluorescence intensity appears a greater leap before and after the induction of IPTG. Also, we induce the improved Lac operon by arabinose to verify its orthogonal response to IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]