Difference between revisions of "Part:BBa K3257044"

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<partinfo>BBa_K3257044 short</partinfo>
 
<partinfo>BBa_K3257044 short</partinfo>
  
Cre recombinase is responsible for recombination of reverse transcribed cDNA, which is also one of the most important processes in our mutation library construction. We expressed Cre recombinase in E.coli and measured its recombination efficiency.
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Cre recombinase is responsible for recombination of reverse transcribed cDNA, which is also one of the most important processes in our mutation library construction. We expressed Cre recombinase in E.coli.
  
  

Revision as of 11:24, 9 September 2019

Cre Recombinase

Cre recombinase is responsible for recombination of reverse transcribed cDNA, which is also one of the most important processes in our mutation library construction. We expressed Cre recombinase in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]