Difference between revisions of "Part:BBa K3257032"
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This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 159th amino acid residual. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing. | This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 159th amino acid residual. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing. | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''Sequence and Features'''</span> |
<partinfo>BBa_K3257032 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3257032 SequenceAndFeatures</partinfo> | ||
Revision as of 10:56, 9 September 2019
EGFP_K159X
This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 159th amino acid residual. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]