Difference between revisions of "Part:BBa K2933102:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | NDM-23 is codon optimized for BL21(DE3) E. coli. | |
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===Source=== | ===Source=== | ||
Latest revision as of 09:07, 8 September 2019
His+Linker a+Sumo+Linker b+SPG-1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 256
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 256
Illegal NheI site found at 33 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 256
Illegal BglII site found at 145
Illegal BamHI site found at 344 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 256
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 256
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
NDM-23 is codon optimized for BL21(DE3) E. coli.
Source
NCBI.