Difference between revisions of "Part:BBa K3020002:Design"
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===Source=== | ===Source=== | ||
| − | + | The eGFP gene sequence used in this experiment was obtained from NCBI, sequence ID: 20473140, and the level of foreign gene expression was affected by the preference of codons.The gene sequence was submitted to Jin Weizhi (Suzhou) Bio Co., Ltd. for codon optimization of E. coli host. | |
| − | The gene sequence was submitted to Jin Weizhi (Suzhou) Bio Co., Ltd. for codon optimization of E. coli host. | + | |
===References=== | ===References=== | ||
Latest revision as of 08:17, 8 September 2019
enhanced Green fluorescent protein reporter gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 682
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
none
Source
The eGFP gene sequence used in this experiment was obtained from NCBI, sequence ID: 20473140, and the level of foreign gene expression was affected by the preference of codons.The gene sequence was submitted to Jin Weizhi (Suzhou) Bio Co., Ltd. for codon optimization of E. coli host.