Difference between revisions of "Part:BBa K2933102"
Line 17: Line 17:
 
<partinfo>BBa_K2933102 parameters</partinfo>
 
<partinfo>BBa_K2933102 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
===Usage and Biology===
 +
This composite part is made up with two basic parts, the His-Sumo tag and our target protein SPG-1. It encodes a protein which is SPG-1 fused with His-Sumo tag. The fusion protein is about 42.2kD. In order to gain the highly purified target protein, we add His-Sumo tag and there is a cutting site of Sumo Proteasein the first part so that the fusion protein can be cut off at the cutting site by Sumo Protease. It is convenient for us to purify our target protein.<br>

Revision as of 03:25, 8 September 2019


His+Linker a+Sumo+Linker b+SPG-1

This part encodes the fusion protein of His-Sumo tag and SPG-1 to promote the expression and purification of target protein(SPG-1).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 256
    Illegal NheI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 256
    Illegal BglII site found at 145
    Illegal BamHI site found at 344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 256
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This composite part is made up with two basic parts, the His-Sumo tag and our target protein SPG-1. It encodes a protein which is SPG-1 fused with His-Sumo tag. The fusion protein is about 42.2kD. In order to gain the highly purified target protein, we add His-Sumo tag and there is a cutting site of Sumo Proteasein the first part so that the fusion protein can be cut off at the cutting site by Sumo Protease. It is convenient for us to purify our target protein.