Difference between revisions of "Part:BBa K2963003"
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<partinfo>BBa_K2963003 short</partinfo> | <partinfo>BBa_K2963003 short</partinfo> | ||
| − | This part contains the pgsB gene for poly-gamma-glutamic acid synthetase. This enzyme is involved in poly-gamma-glutamic synthesis,which converts the glutamate to poly-gamma-glutamic acid. PgsB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we get the pgsB sequenece by error-prone PCR of Bacillus genome, trying to improve the activity of PgsB protein. | + | This part contains the pgsB gene for poly-gamma-glutamic acid synthetase. This enzyme is involved in poly-gamma-glutamic acid synthesis,which converts the glutamate to poly-gamma-glutamic acid. PgsB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we get the pgsB sequenece by error-prone PCR of Bacillus genome, trying to improve the activity of PgsB protein. |
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Revision as of 10:37, 7 September 2019
capB* - a subunit of poly-γ-glutamic acid synthetase
This part contains the pgsB gene for poly-gamma-glutamic acid synthetase. This enzyme is involved in poly-gamma-glutamic acid synthesis,which converts the glutamate to poly-gamma-glutamic acid. PgsB is mainly responsible for catalysing poly-gamma-glutamic acid synthesis. In our subject, we get the pgsB sequenece by error-prone PCR of Bacillus genome, trying to improve the activity of PgsB protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 737
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 737
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 737
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 737
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 737
- 1000COMPATIBLE WITH RFC[1000]