Difference between revisions of "Part:BBa K3257045:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
No.
+
We mutated the 60th nucleotide from C to A, the 61st nucleotide from A to T, 136th nucleotide from C to T, the 490th nucleotide from T to A, the 500th nucleotide from A to G and the 1001st nucleotide from C to G of BBa_C0012.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Bacteria
+
Mutated from BBa_C0012
  
 
===References===
 
===References===
 +
AJ Meyer et al. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26.

Revision as of 17:08, 6 September 2019


Improved LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated the 60th nucleotide from C to A, the 61st nucleotide from A to T, 136th nucleotide from C to T, the 490th nucleotide from T to A, the 500th nucleotide from A to G and the 1001st nucleotide from C to G of BBa_C0012.


Source

Mutated from BBa_C0012

References

AJ Meyer et al. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26.