Difference between revisions of "Part:BBa K3257039"

Revision as of 14:06, 6 September 2019

Chloramphenicol Resistance Gene L158X (ChlR_L158X)

This part is a mutant of a gene encoding ChlR, a resistance gene of chloramphenicol, and it is nonsense mutated at its 158th amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3257039 short</partinfo>
-This part is a mutant of a gene encoding Chl, a resistance gene of chloramphenicol, and it is nonsense mutated at its 158th amino acid residual. We used this mutated Chl as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.
+This part is a mutant of a gene encoding ChlR, a resistance gene of chloramphenicol, and it is nonsense mutated at its 158th amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.
 <!-- Add more about the biology of this part here