Difference between revisions of "Part:BBa K2933021"
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   [[File:Fla.103-PCR.png]]<br>
 
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'''Figure 1.'''  (a) The PCR result of NDM-23. (b) The verification results by enzyme digestion.
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'''Figure 1.'''  (a) The PCR result of Fla.103. (b) The verification results by enzyme digestion.The 1 is the original plasmid.The 2 is the
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Results verified by double enzyme digestion.

Revision as of 13:57, 6 September 2019


subclass B1 metallo-beta-lactamase Fla.103, codon optimized in E. coli

This part encodes a protein called Fla.103, which is a metallo-beta-lactamase of subclass B1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 76
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 558
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Fla.103 is a type of subclass B metal beta-lactamases. The beta lactamases can hydrolyze almost all available beta lactam antibiotics (except aztreonam) clinically, including the broad-spectrum antibiotic carbapenems. Because of the extensive substrate profile of this enzyme, the clinical strains carrying it become a great threat to human life and health.

References

Molecular cloning

Fla.103-PCR.png
Figure 1. (a) The PCR result of Fla.103. (b) The verification results by enzyme digestion.The 1 is the original plasmid.The 2 is the Results verified by double enzyme digestion.