Difference between revisions of "Part:BBa K3257035"

 
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<partinfo>BBa_K3257035 short</partinfo>
 
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This part is a mutant of a gene encoding Chl, a resistance protein of chloramphenicol, and it is nonsense mutated at its 33rd amino acid residual. We used this mutated Chl as one of the targets of our directed evolution process. After expression in BL21(DE3), we found that no colony grew on a medium plate containing chloramphenicol, showing its inability of being resistant to chloramphenicol.
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This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 33rd amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we found that no colony grew on a medium plate containing chloramphenicol, showing its inability of being resistant to chloramphenicol.
  
 
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Revision as of 09:56, 6 September 2019


Chloramphenicol Resistance Gene T33X (ChlR_T33X)

This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 33rd amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we found that no colony grew on a medium plate containing chloramphenicol, showing its inability of being resistant to chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]