Difference between revisions of "Part:BBa K2963000:Design"

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===Design Notes===
 
===Design Notes===
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When we express the CapC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.
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When we express the PgsC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.
  
  

Latest revision as of 08:39, 6 September 2019


PgsC - a submit of poly-gamma-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
    Illegal NgoMIV site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we express the PgsC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.


Source

Bacillus Genome

References