Difference between revisions of "Part:BBa K2933014"
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<partinfo>BBa_K2933014 parameters</partinfo>
 
<partinfo>BBa_K2933014 parameters</partinfo>
 
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===Usage and Biology===
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BcII-194 is a type of subclass B metallo-beta-lactamase found in Bacillus anthracis. It can hydrolyzes Beta-lactam antibiotics to make pathogenic bacteria produce drug resistance. The bacterium Bacillus anthracis is responsible for causing anthrax infection, which is often fatal. Because symptoms arising from the bacterial infection are similar to a common cold,misdiagnosis in the early stage is possible and frequent. So the clinical strains carrying it become a great threat to human life and health. Beta-Lactamase inhibitors such as clavulanic acid, sulbactam, and tazobactam are commercially available and can be used with existing antibiotics to cure some antibiotic resistant infections, but these are not effective against MBLs. Although some potential inhibitors have the ability to inhibit it, there are no commercially available inhibitors of it. We use the protein to carry out high-throughput screening to find inhibitors that can inhibit it.<br>
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==References==
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Sung-Kun Kim, Mara Demuth, Sara R. Schlesinger, Sung Joon Kim, Jonathan Urbanczyk, Robert W. Shaw & Hyunshun Shin (2016) Inhibition of Bacillusanthracis metallo-β- lactamase by compounds with hydroxamic acid functionality, Journal of Enzyme Inhibition and Medicinal Chemistry, 31:sup4, 132-137, DOI: 10.1080/14756366.2016.1222580<br>
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===Molecular cloning===

Revision as of 08:24, 6 September 2019


BCII family subclass B1 metallo-beta-lactamase, codon optimized in E. coli

This part encodes a protein called BcII-194, which is a metallo-beta-lactamase of subclass B1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

BcII-194 is a type of subclass B metallo-beta-lactamase found in Bacillus anthracis. It can hydrolyzes Beta-lactam antibiotics to make pathogenic bacteria produce drug resistance. The bacterium Bacillus anthracis is responsible for causing anthrax infection, which is often fatal. Because symptoms arising from the bacterial infection are similar to a common cold,misdiagnosis in the early stage is possible and frequent. So the clinical strains carrying it become a great threat to human life and health. Beta-Lactamase inhibitors such as clavulanic acid, sulbactam, and tazobactam are commercially available and can be used with existing antibiotics to cure some antibiotic resistant infections, but these are not effective against MBLs. Although some potential inhibitors have the ability to inhibit it, there are no commercially available inhibitors of it. We use the protein to carry out high-throughput screening to find inhibitors that can inhibit it.

References

Sung-Kun Kim, Mara Demuth, Sara R. Schlesinger, Sung Joon Kim, Jonathan Urbanczyk, Robert W. Shaw & Hyunshun Shin (2016) Inhibition of Bacillusanthracis metallo-β- lactamase by compounds with hydroxamic acid functionality, Journal of Enzyme Inhibition and Medicinal Chemistry, 31:sup4, 132-137, DOI: 10.1080/14756366.2016.1222580

Molecular cloning