Difference between revisions of "Part:BBa K2992000"
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<partinfo>BBa_K2992000 short</partinfo> | <partinfo>BBa_K2992000 short</partinfo> | ||
| − | Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST) reporter gene | + | Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST) reporter gene. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:54, 5 September 2019
FAST reporter gene
Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST) reporter gene.
Usage and Biology
FAST is one of the few fluorescent reporters available for effective use in anaerobic organisms. FAST is derived from Halorhodospira halophila and has been codon optimised for fluorescence studies in the genus Clostridium. In our project we use FAST as a reporter to demosntrate the activity of our chosen promoters and to assess its suitability as an alternative non-volatile reporter system for predicting neurotoxin production.
Characterisation
Date incoming
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 155
Characterisation
Date incoming
References
Street et al., 2019 to be updated