Difference between revisions of "Part:BBa K2992001"
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===Usage and Biology===
 
===Usage and Biology===
  
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<p>Pntnh is derived from the nerotoxin gene cluster of <i>C. botulinum</i>. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production.<p>
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<p>Pntnh is derived from the nerotoxin gene cluster of <i>C. botulinum</i>. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production following food processing.  
  
 
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Revision as of 11:37, 5 September 2019


PntnH

Regulatory region comprising promoter 5'-UTR and RBS for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in Clostridum botulinum.

Usage and Biology

Pntnh is derived from the nerotoxin gene cluster of C. botulinum. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production following food processing. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]