Difference between revisions of "Part:BBa K2992001"
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Regulatory region comprising promoter 5'-UTR and RBS for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in <i>Clostridum botulinum</i>. | Regulatory region comprising promoter 5'-UTR and RBS for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in <i>Clostridum botulinum</i>. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p>Pntnh is derived from the nerotoxin gene cluster of <i>C. botulinum</i>. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production.<p> | <p>Pntnh is derived from the nerotoxin gene cluster of <i>C. botulinum</i>. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production.<p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:36, 5 September 2019
PntnH
Regulatory region comprising promoter 5'-UTR and RBS for the non-toxic nonhemmagluttinin component of the neurotoxin gene cluster in Clostridum botulinum.
Usage and Biology
Pntnh is derived from the nerotoxin gene cluster of C. botulinum. This promoter was used to drive expression of of the toxin regulator botR and our chosen reporter genes of interest in order to generate a volatile reporter strain to predict neurotoxin production.<p> Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]