Difference between revisions of "Part:BBa K2963000:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
When we express the CapC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping translation in time.
+
When we express the CapC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
capC sequence can be found in the Bacillus licheniformis
+
Bacillus licheniformis
  
 
===References===
 
===References===

Revision as of 10:48, 5 September 2019


PgsC - a submit of poly-gamma-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
    Illegal NgoMIV site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we express the CapC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.


Source

Bacillus licheniformis

References