Difference between revisions of "Part:BBa K2976010"

 
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K2976010 short</partinfo>
  
 +
===Usage and Biology===
 +
Oligopeptide “2A‐like” sequences are found in a range of RNA virus genomes controlling protein biogenesis and T2A derives from Thosea asigna virus. The 2A peptide consensus motif is extremely rare and is always associated with cleavage activity between the glycine and the proline. The insertion of T2A within a single open reading frame (ORF) produces multiple proteins. Besides, inserting a GSG linker before the T2A sequence significantly improves its self-cleaving efficiency and ensures complete cleavage. In 2019 CPU_CHINA project, we utilize P2A and T2A to co-express TLR1:TLR2:CD14 cluster on our designer cell membrane.
 +
</p>
 +
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K2976010 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K2976010 parameters</partinfo>
 +
<!-- -->

Revision as of 04:59, 5 September 2019

Improved T2A

Usage and Biology

Oligopeptide “2A‐like” sequences are found in a range of RNA virus genomes controlling protein biogenesis and T2A derives from Thosea asigna virus. The 2A peptide consensus motif is extremely rare and is always associated with cleavage activity between the glycine and the proline. The insertion of T2A within a single open reading frame (ORF) produces multiple proteins. Besides, inserting a GSG linker before the T2A sequence significantly improves its self-cleaving efficiency and ensures complete cleavage. In 2019 CPU_CHINA project, we utilize P2A and T2A to co-express TLR1:TLR2:CD14 cluster on our designer cell membrane. </p>


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]