Difference between revisions of "Part:BBa K3113010:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | N55K to get a high affinity mutant | + | N55K to get a high affinity mutant. |
− | V75E and A81G to prevent capsid assembly | + | V75E and A81G to prevent capsid assembly. |
− | + | T3A to remove illegal restriction site. | |
− | + | ||
===Source=== | ===Source=== |
Latest revision as of 16:05, 4 September 2019
MCP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N55K to get a high affinity mutant. V75E and A81G to prevent capsid assembly. T3A to remove illegal restriction site.
Source
This sequence was given to the team by PhD student Christoph Gruber. The sequence originates in the Bacteriophage MS2.