<b>Summary of Results as in https://parts.igem.org/Part:BBa_K2819118</b>
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<ul>
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<li>PhtpG1-mRFP <u><b>can work in different genetic backgrounds</b></u>. Interestingly, the stress reporter was robust in all three strains of <i>E. coli</i> that it was tested in: DH5α, BL21 (DE3) and BL21 Star (DE3).</li>
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<li>PhtpG1-mRFP is <b><u>robust in different temperatures</u></b>. However, at low temperatures i.e. 25°C, more time is expected to allow for growth to stabilize/increase steadily before readings follow an expected trend. Given that PhtpG1 is a promoter that is involved in heat-shock response (and that requires heat-shock response sigma factor (σ32)), higher temperatures would be expected to activate the promoter to a greater extent (Ceroni et al., 2018). </li>
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<li>PhtpG1-mRFP appears to be <b><u>sensitive to the size of constructs that is introduced</u></b> into the cell. The highest levels of mRFP was consistently found to be generated in test construct Set-up C (see Figure 6), in which the large de novo plasmid<sup>a</sup> was expressed, followed by test construct Set-up E and F expressing the moderately sized FNS<sup>a</sup> protein, and the lowest being in test construct Set-up A, in which only GFP was expressed<sup>a</sup>.</li>
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</ul>
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<font size="1"><sup>a</sup>Sizes of proteins: MCS (55 kDa) [de novo plasmid], OsPKS (43 kDa) [de novo plasmid], 4CL (59 kDa) [de novo plasmid], GFP (27 kDa) and FNS (41 kDa).</font><br><br>
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Revision as of 09:00, 31 August 2019
HicA
This part contains the toxin component of a type II toxin-antitoxin (TA) system. HicA is a probable translation-independent mRNA interferase.
