Difference between revisions of "Part:BBa K137076:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K137076=== | ===Applications of BBa_K137076=== | ||
| + | *Strain: This part will only work in a catalase deficient strain of E. coli. Our testing strain, JI377, is DE(katE, katG, ahp). | ||
| + | *Culture conditions: Saturated LB culture backdiluted 1:100 into SOC +1mM IPTG. Let shake at 37C until the culture reaches an OD600 = 0.8. Bring culture to 10nM AHL and let shake at 37 C for 3 hrs. Pellet cells and assay supernatant for hydrogen peroxide. | ||
| + | *Results: Under these conditions the supernatant had ~50 uM H<sub>2</sub>O<sub>2</sub> prior to induction and ~200 uM ~200uM H<sub>2</sub>O<sub>2</sub> 3hrs post induction with AHL. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 07:17, 14 August 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K137076
- Strain: This part will only work in a catalase deficient strain of E. coli. Our testing strain, JI377, is DE(katE, katG, ahp).
- Culture conditions: Saturated LB culture backdiluted 1:100 into SOC +1mM IPTG. Let shake at 37C until the culture reaches an OD600 = 0.8. Bring culture to 10nM AHL and let shake at 37 C for 3 hrs. Pellet cells and assay supernatant for hydrogen peroxide.
- Results: Under these conditions the supernatant had ~50 uM H2O2 prior to induction and ~200 uM ~200uM H2O2 3hrs post induction with AHL.
User Reviews
UNIQ4795d2346ac3721e-partinfo-00000000-QINU UNIQ4795d2346ac3721e-partinfo-00000001-QINU