Difference between revisions of "Part:BBa K2927024"

 
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<partinfo>BBa_K2927024 short</partinfo>
  
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We changed 5 nucleotides of crRNA 1 from 5’ end to make the mutated crRNA 5. It is the sequence for testing whether homemade Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 5 is too short to become the functional protein. There is no concentration for spread out ASFV of our project.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2927024 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2927024 parameters</partinfo>
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Revision as of 05:14, 30 August 2019

Negative control sequence for crRNA 5 target

We changed 5 nucleotides of crRNA 1 from 5’ end to make the mutated crRNA 5. It is the sequence for testing whether homemade Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 5 is too short to become the functional protein. There is no concentration for spread out ASFV of our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]