Difference between revisions of "Part:BBa K2927004"
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TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. It makes Cas12a protein be collected in the solution. Furthermore, the sequence of the TEV cutting site is used by E.coli. | TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. It makes Cas12a protein be collected in the solution. Furthermore, the sequence of the TEV cutting site is used by E.coli. | ||
| + | '''Reference'''<br> | ||
| + | Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 04:46, 29 August 2019
Tobaco Etch Virus (TEV) motif
TEV enzyme cut the TEV cutting site to separate the Cas12a protein from the composition (10X His-MBP-TEV-Cas12a) in our project. It makes Cas12a protein be collected in the solution. Furthermore, the sequence of the TEV cutting site is used by E.coli.
Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]