Difference between revisions of "Part:BBa K2927002"
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__NOTOC__
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<partinfo>BBa_K2927002 short</partinfo>
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10 times histidine is made up of 10 histidines connected. Because of the affinity with Ni, it makes composition (10X His-MBP-TEV-Cas12a) bind on the Ni column. It’s used for Cas12a protein purification. The 10X histidine is used by E.coli.
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'''Reference'''<br>
 
'''Reference'''<br>
 
Janice S. Chen, Enbo Ma, Lucas B. Harrington,  Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018)
 
Janice S. Chen, Enbo Ma, Lucas B. Harrington,  Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018)
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>'''Sequence and Features'''</span><br>
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<partinfo>BBa_K2927002 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2927002 parameters</partinfo>
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Revision as of 04:29, 29 August 2019

10X His

10 times histidine is made up of 10 histidines connected. Because of the affinity with Ni, it makes composition (10X His-MBP-TEV-Cas12a) bind on the Ni column. It’s used for Cas12a protein purification. The 10X histidine is used by E.coli.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]