Difference between revisions of "Part:BBa K2909012:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2909012 short</partinfo>
+
<partinfo>BBa_K2909011 short</partinfo>
  
−
<partinfo>BBa_K2909012 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2909011 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
.
+
 
  
  
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
.
+
The DGAT-1-2 enzyme (BBa_K2909003) comes from the predicted sequence from the E. guineensis genome sequencing.<br>
 +
NCBI Reference Sequence: XM_010933834.1<br>
 +
https://www.ncbi.nlm.nih.gov/nuccore/XM_010933834.1<br><br>
 +
The C-terminal HiBiT tag (BBa_K2909001) comes from Promega (Schwinn et al. 2018).<br><br>
 +
All the other parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018). <br>
  
 
===References===
 
===References===
 +
 +
<ol>
 +
<li> Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018). </li>
 +
<li> Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018). </li>
 +
<li> 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).</li>
 +
</ol>

Latest revision as of 15:34, 28 August 2019

HiBiT-DGAT-1-2_HygroR E. guineensis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 3104
    Illegal PstI site found at 2389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 3104
    Illegal NheI site found at 3368
    Illegal PstI site found at 2389
    Illegal NotI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 3104
    Illegal BglII site found at 2297
    Illegal BamHI site found at 2537
    Illegal XhoI site found at 758
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 3104
    Illegal PstI site found at 2389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 3104
    Illegal PstI site found at 2389
    Illegal NgoMIV site found at 4069
    Illegal NgoMIV site found at 4251
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2575


Design Notes

Source

The DGAT-1-2 enzyme (BBa_K2909003) comes from the predicted sequence from the E. guineensis genome sequencing.
NCBI Reference Sequence: XM_010933834.1
https://www.ncbi.nlm.nih.gov/nuccore/XM_010933834.1

The C-terminal HiBiT tag (BBa_K2909001) comes from Promega (Schwinn et al. 2018).

All the other parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018).

References

  1. Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018).
  2. Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
  3. 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).