Difference between revisions of "Part:BBa K2909008:Design"
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===Source=== | ===Source=== | ||
| − | All parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018). | + | All parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018). <br> |
| + | The LPAAT-A enzyme (BBa_K2909002) comes from the predicted sequence from the E. guineensis genome sequencing.<br> | ||
| + | NCBI Reference Sequence: XM_010933834.1<br> | ||
| + | https://www.ncbi.nlm.nih.gov/nuccore/XM_010933834.1 | ||
===References=== | ===References=== | ||
Revision as of 15:17, 28 August 2019
ParoR_CloverGFP-HiBiT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2806 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal NheI site found at 269
Illegal PstI site found at 2806
Illegal NotI site found at 1779 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal BamHI site found at 11
Illegal XhoI site found at 2320 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2806 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 2806 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3466
Design Notes
Source
All parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018).
The LPAAT-A enzyme (BBa_K2909002) comes from the predicted sequence from the E. guineensis genome sequencing.
NCBI Reference Sequence: XM_010933834.1
https://www.ncbi.nlm.nih.gov/nuccore/XM_010933834.1
References
- Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
- 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).