Difference between revisions of "Part:BBa K2909010"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2909010 short</partinfo>
+
<partinfo>BBa_K29090010 short</partinfo>
  
−
.
 
−
 
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
−
 
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2909010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2909010 SequenceAndFeatures</partinfo>
  
  
−
<!-- Uncomment this to enable Functional Parameter display
+
=='''Introduction'''==
−
===Functional Parameters===
+
 
−
<partinfo>BBa_K2909010 parameters</partinfo>
+
<h3> 1- Biological background </h3>
−
<!-- -->
+
 
 +
This part is a CDS coding for the E. guineensis LPAAT-A enzyme tagged with a C-terminal HiBiT tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Hygromycin resistance cassette called AphVII to select the recombinant clones.<br>
 +
 
 +
This part has been designed to characterize the expression of the E. guineensis LPAAT-A enzyme that we adapted for C. reinhardtii (BBa_K2909002) with the C-terminal HibiT tag.<br>
 +
 
 +
This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI restriciton enzyme. It is this linear DNA that is integrated into the genomic DNA of Chlamydomonas reinhardtii during the transformation.
 +
 
 +
<h3> 2- Usage in iGEM projects </h3>
 +
 
 +
Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

Revision as of 15:09, 28 August 2019

No part name specified with partinfo tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2494
    Illegal PstI site found at 1233
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2494
    Illegal NheI site found at 2758
    Illegal PstI site found at 1233
    Illegal NotI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2494
    Illegal BamHI site found at 1075
    Illegal XhoI site found at 758
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2494
    Illegal PstI site found at 1233
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2494
    Illegal PstI site found at 1233
    Illegal NgoMIV site found at 1788
    Illegal NgoMIV site found at 3459
    Illegal NgoMIV site found at 3641
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1965


Introduction

1- Biological background

This part is a CDS coding for the E. guineensis LPAAT-A enzyme tagged with a C-terminal HiBiT tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Hygromycin resistance cassette called AphVII to select the recombinant clones.

This part has been designed to characterize the expression of the E. guineensis LPAAT-A enzyme that we adapted for C. reinhardtii (BBa_K2909002) with the C-terminal HibiT tag.

This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI restriciton enzyme. It is this linear DNA that is integrated into the genomic DNA of Chlamydomonas reinhardtii during the transformation.

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)