Difference between revisions of "Part:BBa K2909009"

 
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<partinfo>BBa_K2909009 short</partinfo>
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<partinfo>BBa_K290900 short</partinfo>
  
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2909009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2909009 SequenceAndFeatures</partinfo>
  
  
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=='''Introduction'''==
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===Functional Parameters===
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<partinfo>BBa_K2909009 parameters</partinfo>
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<h3> 1- Biological background </h3>
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This part is a CDS coding for the E. guineensis LPAAT-A enzyme tagged with a N-terminal HiBiT tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Hygromycin resistance cassette called AphVII to select the recombinant clones.<br>
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This part has been designed to characterize the expression of the E. guineensis LPAAT-A enzyme that we adapted for C. reinhardtii with the N-terminal HibiT tag(BBa_K2909002).<br>
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This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI restriciton enzyme. It is this linear DNA that is integrated into the genomic DNA of Chlamydomonas reinhardtii during the transformation.
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<h3> 2- Usage in iGEM projects </h3>
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Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

Revision as of 15:08, 28 August 2019

No part name specified with partinfo tag.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2519
    Illegal PstI site found at 1273
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2519
    Illegal NheI site found at 2783
    Illegal PstI site found at 1273
    Illegal NotI site found at 217
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2519
    Illegal BamHI site found at 1115
    Illegal BamHI site found at 1952
    Illegal XhoI site found at 758
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2519
    Illegal PstI site found at 1273
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 2519
    Illegal PstI site found at 1273
    Illegal NgoMIV site found at 1828
    Illegal NgoMIV site found at 3484
    Illegal NgoMIV site found at 3666
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1990


Introduction

1- Biological background

This part is a CDS coding for the E. guineensis LPAAT-A enzyme tagged with a N-terminal HiBiT tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Hygromycin resistance cassette called AphVII to select the recombinant clones.

This part has been designed to characterize the expression of the E. guineensis LPAAT-A enzyme that we adapted for C. reinhardtii with the N-terminal HibiT tag(BBa_K2909002).

This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI restriciton enzyme. It is this linear DNA that is integrated into the genomic DNA of Chlamydomonas reinhardtii during the transformation.

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)