Difference between revisions of "Part:BBa K2909005"
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<h3> 1- Biological background </h3> | <h3> 1- Biological background </h3> | ||
| − | This part is a reporter construct with a Clover GFP reporter gene tagged with a N-terminal NanoLuc under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Paromomycin resistance cassette called AphVIII to select the recombinant clones.<br> | + | This part is a reporter construct with a Clover GFP reporter gene tagged with a N-terminal NanoLuc tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Paromomycin resistance cassette called AphVIII to select the recombinant clones.<br> |
This part has been designed to be used as a control for the characterization of our HiBiT tag parts (BBa_K2909000 and BBa_K2909001).<br> | This part has been designed to be used as a control for the characterization of our HiBiT tag parts (BBa_K2909000 and BBa_K2909001).<br> | ||
Revision as of 14:52, 28 August 2019
ParoR_NanoLuc-CloverGFP
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 3323 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal NheI site found at 269
Illegal PstI site found at 3323
Illegal NotI site found at 1779 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal BglII site found at 2563
Illegal BamHI site found at 11
Illegal BamHI site found at 3930
Illegal XhoI site found at 2320 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 3323 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5
Illegal EcoRI site found at 1567
Illegal PstI site found at 3323
Illegal NgoMIV site found at 2379 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3968
Introduction
1- Biological background
This part is a reporter construct with a Clover GFP reporter gene tagged with a N-terminal NanoLuc tag under the control of a nitrate-inducible Chlamydomonas reinhardtii promoter called NIT1. It also contains a Paromomycin resistance cassette called AphVIII to select the recombinant clones.
This part has been designed to be used as a control for the characterization of our HiBiT tag parts (BBa_K2909000 and BBa_K2909001).
This part is a linear DNA that is the result of the digestion of the level M plasmid by the type IIS BsaI restriciton enzyme. It is this linear DNA that is integrated into the genomic DNA of Chlamydomonas reinhardtii during the transformation.
2- Usage in iGEM projects
Bio[oil]gical Factory (iGEM Sorbonne Université 2019)
Characterization
References
- Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
- 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).