Difference between revisions of "Part:BBa K2959001"

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This composite part enables tha expression of the human antimicrobial peptide LL 37.  The BioBrick consists of parts araC-pBad-rbs-LL37 (BBa_K1230007) and a doulbe terminator (BBa_B0015). The part was designed with the purpose of expressing the peptide in E. coli. Expression of the part is regulated by the pBAD promoter which is inducible through arabinose. The promoter was designed to have low background activty which is ideal for porduction of toxic peptides which is the case of LL 37 production in E. coli. Also, we added part BBa_B0015 in order to create a ready-to-express version of part BBa_K1230007.
 
This composite part enables tha expression of the human antimicrobial peptide LL 37.  The BioBrick consists of parts araC-pBad-rbs-LL37 (BBa_K1230007) and a doulbe terminator (BBa_B0015). The part was designed with the purpose of expressing the peptide in E. coli. Expression of the part is regulated by the pBAD promoter which is inducible through arabinose. The promoter was designed to have low background activty which is ideal for porduction of toxic peptides which is the case of LL 37 production in E. coli. Also, we added part BBa_B0015 in order to create a ready-to-express version of part BBa_K1230007.
  
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===Usage and Biology===
 
===Usage and Biology===
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The protein LL-37 cathelicidin is a human antimicrobial peptide active against Gram-positive bacteria, Gram-negative bacteria and fungi<sup>1</sup>. LL-37 is the only known cathelicidin peptide produced in humans; its structure is amphipathic and has an α-helical conformation with a positive charge. Talking about its mechanism of action, the cathelicidins targeted the cell membrane and causes its complete disruption inducing cell membrane permeabilization and simultaneous vacuolar expansion. Intracellular activities of the peptide may contribute to its antifungal activity<sup>2</sup>.
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
 
<partinfo>BBa_K2959001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2959001 SequenceAndFeatures</partinfo>
  
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===References===
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1. Tollin, M. & Bergman, P. (2003). Antimicrobial peptides in the first line defence of human colon mucosa. (4):523-30 DOI:10.1016/s0196-9781(03)00114-1
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2. Ordonez, S. R., Amarullah, I. H., Wubbolts, R. W., Veldhuizen, E. J., & Haagsman, H. P. (2014). Fungicidal mechanisms of cathelicidins LL-37 and CATH-2 revealed by live-cell imaging. Antimicrobial agents and chemotherapy, 58(4), 2240-2248. DOI: 10.1128/AAC.01670-13
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Latest revision as of 04:02, 28 August 2019


Expressible LL 37 with Arabinose Inducible Promoter

This composite part enables tha expression of the human antimicrobial peptide LL 37. The BioBrick consists of parts araC-pBad-rbs-LL37 (BBa_K1230007) and a doulbe terminator (BBa_B0015). The part was designed with the purpose of expressing the peptide in E. coli. Expression of the part is regulated by the pBAD promoter which is inducible through arabinose. The promoter was designed to have low background activty which is ideal for porduction of toxic peptides which is the case of LL 37 production in E. coli. Also, we added part BBa_B0015 in order to create a ready-to-express version of part BBa_K1230007.

Usage and Biology

The protein LL-37 cathelicidin is a human antimicrobial peptide active against Gram-positive bacteria, Gram-negative bacteria and fungi1. LL-37 is the only known cathelicidin peptide produced in humans; its structure is amphipathic and has an α-helical conformation with a positive charge. Talking about its mechanism of action, the cathelicidins targeted the cell membrane and causes its complete disruption inducing cell membrane permeabilization and simultaneous vacuolar expansion. Intracellular activities of the peptide may contribute to its antifungal activity2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

References

1. Tollin, M. & Bergman, P. (2003). Antimicrobial peptides in the first line defence of human colon mucosa. (4):523-30 DOI:10.1016/s0196-9781(03)00114-1

2. Ordonez, S. R., Amarullah, I. H., Wubbolts, R. W., Veldhuizen, E. J., & Haagsman, H. P. (2014). Fungicidal mechanisms of cathelicidins LL-37 and CATH-2 revealed by live-cell imaging. Antimicrobial agents and chemotherapy, 58(4), 2240-2248. DOI: 10.1128/AAC.01670-13