Difference between revisions of "Part:BBa K2959000"
Alejandro ah (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
This composite part enables tha expression of the human antimicrobial peptide LL 37 in E. coli BL21 (DE3). The BioBrick consists of a T7 Promoter + RBS + LL 37 sequence + double terminator (rrnBT1 + T7TE). The T7 promoter, inducible through IPTG, controls the expression of the part. | This composite part enables tha expression of the human antimicrobial peptide LL 37 in E. coli BL21 (DE3). The BioBrick consists of a T7 Promoter + RBS + LL 37 sequence + double terminator (rrnBT1 + T7TE). The T7 promoter, inducible through IPTG, controls the expression of the part. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | <p align="justify"> | |
| − | + | Defensin 1 is an antimicrobial peptide of the honeybee. It's a component of their innate immune response, which contains 51 amino acids and 6 cysteine residues forming three disulfide bonds<sup>2</sup>. It is synthesized in salivary glands and characterizes the social immunity. Sometimes is able to protect honeybees even in early stages and act as part of individual immunity<sup>4</sup>. This AMP is expressed in the head and thorax of honey bees by the hypopharyngeal, mandibular and thoracic salivary glands<sup>3</sup>. It is present in the royal jelly, honey and hemolymph. Originally, it was isolated from royal jelly, and therefore named royalisin<sup>2</sup>. | |
| + | <br> | ||
<!-- --> | <!-- --> | ||
Revision as of 23:23, 27 August 2019
Expressible LL 37 - Cathelicidin
This composite part enables tha expression of the human antimicrobial peptide LL 37 in E. coli BL21 (DE3). The BioBrick consists of a T7 Promoter + RBS + LL 37 sequence + double terminator (rrnBT1 + T7TE). The T7 promoter, inducible through IPTG, controls the expression of the part.
Usage and Biology
Defensin 1 is an antimicrobial peptide of the honeybee. It's a component of their innate immune response, which contains 51 amino acids and 6 cysteine residues forming three disulfide bonds2. It is synthesized in salivary glands and characterizes the social immunity. Sometimes is able to protect honeybees even in early stages and act as part of individual immunity4. This AMP is expressed in the head and thorax of honey bees by the hypopharyngeal, mandibular and thoracic salivary glands3. It is present in the royal jelly, honey and hemolymph. Originally, it was isolated from royal jelly, and therefore named royalisin2.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]