Difference between revisions of "Part:BBa K2973009:Design"
Nickdelkis (Talk | contribs) (→References) |
|||
| Line 16: | Line 16: | ||
===References=== | ===References=== | ||
| + | D Thierry, A Brisson-Noël, V Vincent-Lévy-Frébault, S Nguyen, J L Guesdon, B Gicquel, Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis (1990), Journal of Clinical Microbiology | ||
| + | |||
| + | Thabet, S., & Souissi, N. (2016). Transposition mechanism, molecular characterization and evolution of IS6110, the specific evolutionary marker of Mycobacterium tuberculosis complex. Molecular Biology Reports, 44(1), 25–34.doi:10.1007/s11033-016-4084-x | ||
Revision as of 22:15, 26 August 2019
IS6110 (Mycobacterium tuberculosis)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 880
Illegal XhoI site found at 1266 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 232
Illegal NgoMIV site found at 1305 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1314
Illegal BsaI.rc site found at 1295
Design Notes
NCBI Mycobacterium tuberculosis Genome, Strain H37Rv, Accession Number: NC_000962
Source
IS6110 has normally two open reading frames. OrfA (327 bp) and orfB (927 bp) are consecutive ORFs partially overlapping in the relative translational reading frames 0 and −1, respectively. For the purposes of our project, we designed primers for orfB only.
References
D Thierry, A Brisson-Noël, V Vincent-Lévy-Frébault, S Nguyen, J L Guesdon, B Gicquel, Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis (1990), Journal of Clinical Microbiology
Thabet, S., & Souissi, N. (2016). Transposition mechanism, molecular characterization and evolution of IS6110, the specific evolutionary marker of Mycobacterium tuberculosis complex. Molecular Biology Reports, 44(1), 25–34.doi:10.1007/s11033-016-4084-x