Difference between revisions of "Part:BBa K3182300"
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===Usage and Biology===
 
===Usage and Biology===
  
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[[File:T--Linkoping_Sweden--mngphoto.png|900px|thumb|center|<b>Figure 3.</b> Benchling screenshot of the expression system. The T7-RNA-polymerase promotor is followed by a T7 g10 leader sequence which enhances the binding to the 16S ribosomal RNA. After the leader sequence a poly A spacer is found, which has been shown to increase translation in vitro. Before the start codon a strong RBS, g10-L, followed by an AT-rich spacer can be seen, which will slightly increase translation of the following gene.]]
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[[File:T--Linkoping_Sweden--mngphoto.png|900px|thumb|center|<b>Figure 3.</b> mNG induced]]
  
 
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Revision as of 20:12, 26 August 2019


pT7-mNG

mNeonGreen with a T7 promotor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 91
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]