Difference between revisions of "Part:BBa K2909003"
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It catalyzes the incorporation of the third and last fatty acid at the position sn-3 of the glycerol in the triglyceride synthesis (Kennedy) pathway. | It catalyzes the incorporation of the third and last fatty acid at the position sn-3 of the glycerol in the triglyceride synthesis (Kennedy) pathway. | ||
| − | The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends | + | The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends. |
| − | + | ||
| + | The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5. | ||
<h3> 2- Usage in iGEM projects </h3> | <h3> 2- Usage in iGEM projects </h3> | ||
Revision as of 17:13, 26 August 2019
DGAT-1-2-B3-B4 E. guineensis codon optimized for C. reinhardtii
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 162
Illegal PstI site found at 1590 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 162
Illegal PstI site found at 1590
Illegal NotI site found at 400 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1498
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 162
Illegal PstI site found at 1590 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 162
Illegal PstI site found at 1590
Illegal NgoMIV site found at 531
Illegal NgoMIV site found at 624 - 1000COMPATIBLE WITH RFC[1000]
Introduction
1- Biological background
Coding sequence of the DGAT-1-2 enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.
It catalyzes the incorporation of the third and last fatty acid at the position sn-3 of the glycerol in the triglyceride synthesis (Kennedy) pathway.
The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends.
The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.
2- Usage in iGEM projects
Bio[oil]gical Factory (iGEM Sorbonne Université 2019)
Characterization
References
- Dussert, S. et al. Comparative Transcriptome Analysis of Three Oil Palm Fruit and Seed Tissues That Differ in Oil Content and Fatty Acid Composition. PLANT PHYSIOLOGY 162, 1337–1358 (2013).
- Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
- 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).