Difference between revisions of "Part:BBa K2909002"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2909002 short</partinfo>
 
<partinfo>BBa_K2909002 short</partinfo>
  
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Coding sequence of the LPAAT-A enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.
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<span class='h3bb'>Sequence and Features</span>
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It catalyzes the incorporation of the second fatty acid at the position sn-2 of the glycerol in the triglyceride synthesis (Kennedy) pathway.
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<partinfo>BBa_K2909002 SequenceAndFeatures</partinfo>
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The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position.
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=='''Introduction'''==
  
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<!-- Add more about the biology of this part here
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<h3> 1- Biological background </h3>
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===Usage and Biology===
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<!-- -->
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Coding sequence of the LPAAT-A enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.<br>
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<span class='h3bb'>Sequence and Features</span>
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It catalyzes the incorporation of the second fatty acid at the position sn-2 of the glycerol in the triglyceride synthesis (Kennedy) pathway.<br>
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<partinfo>BBa_K2909002 SequenceAndFeatures</partinfo>
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The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position.
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<h3> 2- Usage in iGEM projects </h3>
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Bio[oil]gical Factory (iGEM Sorbonne Université 2019)
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=='''Characterization'''==
  
  
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<!-- Uncomment this to enable Functional Parameter display
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=='''References'''==
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===Functional Parameters===
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<partinfo>BBa_K2909002 parameters</partinfo>
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Revision as of 16:12, 26 August 2019

LPAAT-A-B3-B4 E. guineensis codon optimized for C. reinhardtii

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 474
    Illegal NgoMIV site found at 204
    Illegal NgoMIV site found at 1029
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

1- Biological background

Coding sequence of the LPAAT-A enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.
It catalyzes the incorporation of the second fatty acid at the position sn-2 of the glycerol in the triglyceride synthesis (Kennedy) pathway.
The CDS is standardized to the C. reinhardtii MoClo Kit for the B3-B4 position.

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

Characterization

References