Difference between revisions of "Part:BBa K2909001"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2909001 short</partinfo>
 
<partinfo>BBa_K2909001 short</partinfo>
  
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HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence.
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<span class='h3bb'>Sequence and Features</span>
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This part is standardized in the MoClo standard for Chlamydomonas reinhardtii.  
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<partinfo>BBa_K2909001 SequenceAndFeatures</partinfo>
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This tag is flanked on both side by specific fusion sites and BbsI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit.
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=='''Introduction'''==
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<h3> 1- Biological background </h3>
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HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence (https://www.promega.com/resources/pubhub/features/hibit-a-tiny-tag-for-antibody-free-endogenous-protein-detection/).<br>
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This part is standardized in the MoClo standard for Chlamydomonas reinhardtii.<br>
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This tag is flanked on both side by specific fusion sites and BbsI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit.<br>
 
This tag is designed to be integrated at the B5 position (C-terminal tag).
 
This tag is designed to be integrated at the B5 position (C-terminal tag).
  
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<!-- Add more about the biology of this part here
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<h3> 2- Usage in iGEM projects </h3>
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===Usage and Biology===
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<!-- -->
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Bio[oil]gical Factory (iGEM Sorbonne Université 2019)
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2909001 SequenceAndFeatures</partinfo>
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=='''Characterization'''==
  
  
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<!-- Uncomment this to enable Functional Parameter display
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=='''References'''==
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===Functional Parameters===
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<ol>
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<partinfo>BBa_K2909001 parameters</partinfo>
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<li> Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018). </li>
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<!-- -->
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<li> Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018). </li>
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<li> 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).
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</li>
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</ol>

Revision as of 15:15, 26 August 2019

HiBiT-B5 MoClo C. reinhardtii

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

1- Biological background

HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence (https://www.promega.com/resources/pubhub/features/hibit-a-tiny-tag-for-antibody-free-endogenous-protein-detection/).
This part is standardized in the MoClo standard for Chlamydomonas reinhardtii.
This tag is flanked on both side by specific fusion sites and BbsI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit.
This tag is designed to be integrated at the B5 position (C-terminal tag).

2- Usage in iGEM projects

Bio[oil]gical Factory (iGEM Sorbonne Université 2019)

Characterization

References

  1. Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018).
  2. Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
  3. 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).