Difference between revisions of "Part:BBa K2909000"
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<h3> 1- Biological background </h3> | <h3> 1- Biological background </h3> | ||
| − | HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence. | + | HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence (https://www.promega.com/resources/pubhub/features/hibit-a-tiny-tag-for-antibody-free-endogenous-protein-detection/). |
This part is standardized in the MoClo standard for Chlamydomonas reinhardtii. | This part is standardized in the MoClo standard for Chlamydomonas reinhardtii. | ||
This tag is flanked on both side by specific fusion sites and BbsI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit. | This tag is flanked on both side by specific fusion sites and BbsI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit. | ||
Revision as of 15:08, 26 August 2019
HiBiT-B2 MoClo C. reinhardtii
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
1- Biological background
HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence (https://www.promega.com/resources/pubhub/features/hibit-a-tiny-tag-for-antibody-free-endogenous-protein-detection/). This part is standardized in the MoClo standard for Chlamydomonas reinhardtii. This tag is flanked on both side by specific fusion sites and BbsI sites to allow its integration into a level 0 plasmid of the C. reinhardtii MoClo Kit. This tag is designed to be integrated at the B2 position (N-terminal tag).
2- Usage in iGEM projects
Bio[oil]gical Factory (iGEM Sorbonne Université 2019)